Optimization of root system architecture (RSA) is an important objective for modern tomato breeding. However, our knowledge about the mechanisms involved in RSA regulation and salt avoidance in tomato is still scarce because most of the studies were conducted in Arabidopsis. Thus, exploiting genomic resources, tools, candidate genes and the integration of the knowledge from Arabidopsis to tomato, are remaining challenges as there is no well-established system for assessing the RSA of tomato plants. Therefore, this research will develop methods and tools for genome-wide association studies (GWAS) for RSA in tomato. From this study, I am aiming to identify genetic loci that are regulating tomato survivals and unravel the molecular mechanism for tomato RSA regulation in salinized environments.